Abstract: Drought stress has a serious impact on the growth and development of cotton. To explore the relevant molecular mechanism of the drought stress response in cotton, gene mapping based on the OTL interval mapped by simplified genome BSA-seg of the drought-resistance-related RⅠL, population was performed. A QTL regionspanning 2.02 Mb on chromosome D07 was selected, and 201 resource materials were genotyped using 9 KASP markers in the interval. After local interval haplotype association analysis, the overlap of the 110 kb peak regionconfirmed the reliability of this region, and at the same time, the role of GhGF14-30, the only gene in the overlapping region, was modeled in the response of cotton to drought stress. qRT-PCR analysis of the materialsand population parents proved that this gene plays a role in the drought stress response in cotton. Virus-inducedgene silencing proved the importance of this gene in drought-sensitive materials, and drought-resistance-relatedmarker genes also proved that the GhGF14-30 gene may play an important role in the ABA and SOS signaling pathways, This study provides a basis for mining drought stress response functional genes in cotton and lays the foundation for the molecular mechanism of the GhGF14-30 gene in response to drought stress in cotton.
Keywords: Cotton, Drought stress response, Kompetitive allele-specific PCR (KASP), Local interval haplotype association analysis
